Gene expression analysis by cDNA microarray comparing P. monodon transcripts between testis (TT) and ovary (OV) in individual juvenile (Ji), pooled juvenile (Jp) and individual wild-caught broodstock (WBi). TT was labeled with Cy5 fluorescent dye (red) and OV with Cy3 fluorescent dye (green). (A) Hierarchical clustering analysis of the transcripts present in at least 8 of 9 slides and whose expression differences were at least equal to median value ± 1SD in at least 1 slide. (B) Clusters I and II of transcripts with higher expression levels in testis than ovary and vice versa with at least 3-fold difference in at least 6 of 9 slides. (C) Examples of differentially expressed transcripts with putative functions in reproductive development. Transcripts in blue letters are those which were not found in any EST libraries of other tissues. Saposin and Dmc1 (in red) were further characterized by RACE-PCR. (D) Library distributions of transcripts expressed higher in ovary than in testis (244 transcripts) and (E) those expressed higher in testis than in ovary (239 transcripts).