Skip to main content
Figure 3 | BMC Molecular Biology

Figure 3

From: The YEATS family member GAS41 interacts with the general transcription factor TFIIF

Figure 3

Specific binding of GAS41 to TFIIF. HEK293 cells were transfected with pSG5-HA-GAS41 or pSG5. (A) Expression of HA-GAS41, RNA polymerase II, TBP, RAP30, and ACTB was confirmed by immunoblot. Immunoprecipitation of HA-GAS41 and immunoblot disprove a direct interaction of GAS41 and TBP or RNA polymerase II in vivo. (B) GST-GAS41 was incubated with HeLa nuclear extract as prey and subjected to extensive washing steps with increasing salt concentration. Immunoblot confirmed the interaction of GST-GAS41 and RAP30 up to 0.5 mM NaCl.

Back to article page