Figure 2
From: The YEATS family member GAS41 interacts with the general transcription factor TFIIF
GAS41 and TFIIF interact in vivo. For co-immunoprecipitation HEK293 cells were co-transfected with pSG5-HA-GAS41 and p3xFLAG-CMV10-RAP30. (A) Expression of HA-GAS41 and FLAG-RAP30 was confirmed by immunoblot. (B) Immunoprecipitation of FLAG-RAP30 revealed the in vivo interaction of GAS41 and RAP30. (C) Immunoprecipitation of HA-GAS41 and immunoblot using FLAG antibody confirmed this in vivo interaction. A weak background signal of FLAG-RAP30 shows in lane 3. (D) Co-immunoprecipitation of endogenous GAS41, RAP30, and RAP74 from HeLa nuclear extracts confirmed the interaction of GAS41 and TFIIF. Cytoplasmic extract was used as negative control (input). For immunoprecipitation, HeLa nuclear extracts were subjected to protein A-sepharose coupled with either GAS41 antibodies or isotype control.