Figure 1
From: The YEATS family member GAS41 interacts with the general transcription factor TFIIF
GAS41 directly interacts with TFIIF. (A) GST-GAS41 and GST were purified and subjected to SDS-PAGE. Coomassie staining (left) and immunoblot using GAS41 antibody (right), identified GST-GAS41 and GST. (B) GST pull-down assay with GST-GAS41 as bait retained His-RAP30, while negative control GST did not. His-RAP74 was retained by GST-GAS41 as bait, but not by GST as negative control. (C) GST pull-down assay using HeLa nuclear extract confirms the in vitro interaction of GST-GAS41 with both, RAP30 and RAP74.