Table 1 Comprehensive list of oligonucleotide primers used for RACE and quantitative PCR
From: Characterisation of the sarcomeric myosin heavy chain multigene family in the laboratory guinea pig
Primer Name | Forward (5' - 3') | Reverse (5' - 3') | Tm (°C) | Expected Product (bp) |
|---|---|---|---|---|
GP_626 bp | TGTCATCCAGTACTTTGCAACAA | TGAGCAGGTCAGCAGAGTTC | 60 | 626 |
Reverse_GSP_2 | - | CAGGCTGCGTAACGCTCTTTGAGGTTGT | 68 | - |
Reverse_Nested_GSP_2 | - | CTCGTGCAGGTGGGTCATCATGG | 68 | - |
Forward_GSP_2 | CATGATGGCCGAGGAGCTGAAGAAG | - | 68 | - |
Forward_Nested_GSP_2 | AAGGGCGGCAAGAAGCAGATCCAGAAG | - | 68 | - |
MyH7Term3' | AAGTATCGCAAGGCTCAA | CCTTTCCTTAATTCCAAGC | 55 | 129 |
MyH13'UTR | TTCATCCAAATGCAGGAAAG | TCTTTATCTCAAAAGTCATAAATACAA | 55 | 90 |
MyH23'UTR | TGTGGAATGACCAGAGCAAG | CCTTTGCAATAGGGTAGGACA | 55 | 85 |
MyH43'UTR | TCCATCTACTGCTGCAACG | ACTCTGCAGATTTTATTTCCTTG | 55 | 93 |