Figure 4
From: Characterisation of the sarcomeric myosin heavy chain multigene family in the laboratory guinea pig
Specificity of quantitative PCR reactions for each target MHC gene transcript. Linearised vectors containing the 3' UTRs pertaining to MyH1 (A), 2 (B) and 4 (C) were adjusted to 2 ng/μl and serially diluted. Five-fold serial dilutions were then mixed 1:1 (vol/vol) with molecular biology grade water (control) or a combination of the two competing vectors at a concentration of 0.4 ng/μl and subjected to quantitative PCR with the respective MyH primer sets in triplicate. No cross-reaction was noted between any of the MyH primer sets and competing vectors. Black line - control reaction; Grey line - reaction with competitor vectors added; Axes - Log 10 vector concentration against crossing point (Cp); Dashed line - predicted observation should cross-reaction occur.