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Table 6 Results of the gene expression analysis with REST for aTub, GAPDH and MMP with different normalization strategies

From: Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Normalized against Target gene x-fold change in gene expression* SE range P
geNorm: Tbp, Xbp1, Capon aTub 0.62 0.50 - 1.02 0.009
  GAPDH 0.60 0.32 - 1.06 0.007
  MMP 2.64 1.06 - 7.18 0.003
NormFinder I: Tbp, Xbp1 aTub 0.61 0.51 - 0.97 0.001
  GAPDH 0.59 0.33 - 0.97 0.002
  MMP 2.60 0.90 - 7.68 0.005
NormFinder II: Xbp1, Stx16 aTub 0.71 0.51 - 1.13 0.034
  GAPDH 0.69 0.44 - 1.08 0.013
  MMP 3.02 1.05 - 9.80 0.003
Classical HKGs: Tbp, aTub, GAPDH MMP 3.66 1.00 - 13.16 0.003
All genes MMP 3.27 1.11 - 11.07 0.002
  1. *x-fold change in gene expression in the induced samples compared to the control group.