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Table 6 Results of the gene expression analysis with REST for aTub, GAPDH and MMP with different normalization strategies

From: Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Normalized against

Target gene

x-fold change in gene expression*

SE range

P

geNorm: Tbp, Xbp1, Capon

aTub

0.62

0.50 - 1.02

0.009

 

GAPDH

0.60

0.32 - 1.06

0.007

 

MMP

2.64

1.06 - 7.18

0.003

NormFinder I: Tbp, Xbp1

aTub

0.61

0.51 - 0.97

0.001

 

GAPDH

0.59

0.33 - 0.97

0.002

 

MMP

2.60

0.90 - 7.68

0.005

NormFinder II: Xbp1, Stx16

aTub

0.71

0.51 - 1.13

0.034

 

GAPDH

0.69

0.44 - 1.08

0.013

 

MMP

3.02

1.05 - 9.80

0.003

Classical HKGs: Tbp, aTub, GAPDH

MMP

3.66

1.00 - 13.16

0.003

All genes

MMP

3.27

1.11 - 11.07

0.002

  1. *x-fold change in gene expression in the induced samples compared to the control group.