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Table 5 Most stable reference genes and optimum number of reference genes calculated by geNorm and NormFinder

From: Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

 

geNorm

NormFinder

  

1stanalysis

2ndanalysis

 

Tbp/CAPON

Xbp1

Xbp1

 

Xbp1

Tbp

Tbp

 

Stx16

CAPON

CAPON

 

GAPDH

Stx16

Stx16

 

aTub

aTub

 
 

MMP

GAPDH

 
  

MMP

 

Optimum number

3

2

2

  1. Genes are ranked according to Figure 1A (geNorm) and to their stability values in Table 3 and 4, respectively (NormFinder). In bold letters: The recommended optimum combination of genes.