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Table 2 Descriptive statistic analysis with BestKeeper

From: Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Gene aTub GAPDH Tbp Stx16 Xbp1 MMP CAPON
N 24 24 24 24 24 24 24
GM [CP] 20.83 19.7 27.66 26.88 26.41 28.89 27.73
AM [CP] 20.87 19.72 27.68 26.9 26.44 28.95 27.74
Min [CP] 19.19 18.67 26.74 25.49 24.81 24.63 26.43
Max [CP] 26.84 23.58 30.16 30.71 29.14 33.67 30.21
SD [CP] 0.82 0.65 0.65 0.77 0.86 1.56 0.71
CV [% CP] 3.92 3.29 2.37 2.86 3.24 5.37 2.54
Min [x-fold] -2.68 -1.89 -1.73 -2.36 -2.69 -13.75 -2.26
Max [x-fold] 36.98 10.96 4.4 10.6 5.43 18.92 4.72
SD [± x-fold] 1.62 1.47 1.47 1.58 1.66 2.51 1.52
BK Corr [r] 0.875 0.84 0.952 0.952 0.978 0.751 0.884
p-value 0.001 0.001 0.001 0.001 0.001 0.001 0.001
BK Corr -MMP [r] 0.943 0.917 0.922 0.982 0.944   0.803
p-value 0.001 0.001 0.001 0.001 0.001 0.001 0.001
  1. Abbreviations: N: number of samples; GM [CP]: the geometric mean of CP; AM [CP]: the arithmetic mean of CP; Min [CP] and Max [CP]: the extreme values of CP; SD [CP]: the standard deviation of the CP; CV [% CP]: the coefficient of variance expressed as a percentage on the CP level; Min [x-fold] and Max [x-fold]: the extreme values of expression levels expressed as an absolute x-fold over- or under-regulation coefficient; SD [± x-fold]: standard deviation of the absolute regulation coefficients; BK CorrC [r]: Pearson correlation coefficient, correlation between the BestKeeper index and the contributing gene. BK Corr -MMP [r]: Pearson correlation coefficient between BestKeeper index without MMP in the analysis and the contributing gene.