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Table 2 Descriptive statistic analysis with BestKeeper

From: Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Gene

aTub

GAPDH

Tbp

Stx16

Xbp1

MMP

CAPON

N

24

24

24

24

24

24

24

GM [CP]

20.83

19.7

27.66

26.88

26.41

28.89

27.73

AM [CP]

20.87

19.72

27.68

26.9

26.44

28.95

27.74

Min [CP]

19.19

18.67

26.74

25.49

24.81

24.63

26.43

Max [CP]

26.84

23.58

30.16

30.71

29.14

33.67

30.21

SD [CP]

0.82

0.65

0.65

0.77

0.86

1.56

0.71

CV [% CP]

3.92

3.29

2.37

2.86

3.24

5.37

2.54

Min [x-fold]

-2.68

-1.89

-1.73

-2.36

-2.69

-13.75

-2.26

Max [x-fold]

36.98

10.96

4.4

10.6

5.43

18.92

4.72

SD [± x-fold]

1.62

1.47

1.47

1.58

1.66

2.51

1.52

BK Corr [r]

0.875

0.84

0.952

0.952

0.978

0.751

0.884

p-value

0.001

0.001

0.001

0.001

0.001

0.001

0.001

BK Corr -MMP [r]

0.943

0.917

0.922

0.982

0.944

 

0.803

p-value

0.001

0.001

0.001

0.001

0.001

0.001

0.001

  1. Abbreviations: N: number of samples; GM [CP]: the geometric mean of CP; AM [CP]: the arithmetic mean of CP; Min [CP] and Max [CP]: the extreme values of CP; SD [CP]: the standard deviation of the CP; CV [% CP]: the coefficient of variance expressed as a percentage on the CP level; Min [x-fold] and Max [x-fold]: the extreme values of expression levels expressed as an absolute x-fold over- or under-regulation coefficient; SD [± x-fold]: standard deviation of the absolute regulation coefficients; BK CorrC [r]: Pearson correlation coefficient, correlation between the BestKeeper index and the contributing gene. BK Corr -MMP [r]: Pearson correlation coefficient between BestKeeper index without MMP in the analysis and the contributing gene.