Figure 4

Western blotting of acetylated histone H4 . HeLa cells were treated with 8 μM cisplatin, with 5 mM butyrate, or with both. Four and 24 hours after the treatment aliquots were withdrawn, total histone fraction was isolated and equal amount of histones were subjected to SDS-polyacrylamide gel electrophoresis. (A) The proteins were transferred onto nitrocellulose membranes and stained with anti-acetylated histone H4 antibody. Ponceau S staining of H4 is shown as loading control. (B) The acetylated histone H4 was visualized and quantified by the Odyssey scanning system. The acetylation levels are expressed as percentage of the untreated control. The results are means of five independent experiments and standard deviations are shown by error bars.