Figure 5

Second-strand synthesis by PCR reaction. A)Determination of the optimal PCR amplification cycle: the 2% agarose gel shows the amplification products of RH30 first-strand cDNA stopped at 15, 20, 25 and 30 PCR cycles. B) Presence of specific miRNAs in the labeled cDNA population: the 2.25% agarose gel shows PCR amplification products of three specific miRNAs (miR-1, miR-206 and miR-450) performed by using the sequence of each miRNA as forward primer, and the sequence for T7 promoter, which is common to all labeled molecules, as reverse primer.