Figure 2

Specificity of hybridization between capture probes and miRNA targets. Biotynilated miR-199a and miR27a (bottom panel) were captured in solution by the corresponding probes with exactly matching sequences (light grey boxes) or with one (dark grey boxes) or two mismatched bases (black boxes). The fluorescence signal obtained by the hybridization of both the perfectly matching probes was set to 100%. The hybridization signal between both capture probes and the corresponding miRNA target shows a statistically significant decrease (p-value < 0.05) when one or two mismatches are introduced into the capture sequence. Hybridization experiments were obtained with 18.75 fmol of biotynilated targets that fall in the linearity range of our method as shown in Figure 1B. Mean value of expression and 95% confidence intervals are associated to each fluorescence value (%MFI corresponds to the percentage of Median Fluorescence Intensity).