Figure 1

Stability of conjugated microspheres and sensitivity of hybridization. A) Increasing amounts of miR-199a target oligonucleotide (from 15 amol to 300 fmol) show higher fluorescence levels reaching a plateau when the target is present at high concentrations. The curve shows a logarithmic trend. We observed a slightly decrease of the hybridization signal at 50, 65 and 80 days after conjugation. B) Calibration curve (0-35 days) of miR-199a in a log-scale from 15 amol to 300 fmol. The two lines show the linear and plateau range of fluorescence levels representing by two different slopes: 0.94 and 0.15 respectively with R² = 0.99. The linearity range of our technique is comprised between a lower value of 0.073 fmol and an upper value of 18.75 fmol. Mean value of expression and 95% confidence intervals are associated to each fluorescence value (MFI corresponds to Median Fluorescence Intensity).