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Table 2 Relative binding efficiencies of LrpA1 to sepharose-bound DNA fragments

From: Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1

DNA-fragment

band density

% a

lrpA1-Pincl

846 ± 280

100%

lrpA1-Pincl(mutated)

201 ± 33

24%

control-DNA (C)

313 ± 70

37%

aspB3-Pincl (+ 5 mM asp)

1404 ± 228

100%

aspB3-Pincl

473 ± 220

34%

control-DNA (+ 5 mM asp) (C)

92 ± 33

7%

aspB3-Pincl (+5 mM arg)

175 ± 94

100%

aspB3-Pincl

52 ± 22

77%

control-DNA (+ 5 mM arg) (C)

135 ± 69

30%

  1. a Average and standard deviation values are based on three separate experiments