Figure 4
From: Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1
Effects of LrpA1 binding to promoter fragments. Analytical DNA-affinity chromatography showing binding of LrpA1 to DNA fragments (lrpA1-Pincl, aspB3-Pincl and control DNA flgB1) analyzed by SDS-PAGE. The lower band on the gel shows the monomer of LrpA1, whereas the upper band is the dimeric form. Binding of LrpA1 to non specific DNA control (flgB1) (lane 1), to lrpA1-Pincl (lane 2) and to mutated lrpA1-Pincl (lane 3), with an altered inverted repeat sequence (see Fig. 3A) (A). LrpA1 binding to non specific control DNA (flgB1) in the presence of 5 mM L-aspartate (lane 1), to the aspB3-Pincl (lane 2) and to the aspB3-Pincl in the presence of 5 mM L-aspartate (B). Binding of LrpA1 to non specific DNA (flgB1) with 5 mM L-arginine (lane 1) and to the aspB3-Pincl without (lane 2) and with (lane 3) 5 mM L-arginine (C).