Table 5 Primer sequences of eight reference genes used in the validation study.
From: Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples
Target Gene | Gene Accession Number | Tm | Primer Sequence |
|---|---|---|---|
B2M | NM_004048.2 | 55°C | F-5'GTGCTCGCGCTACTACTCTCTCT R-5'TCAATGTCGGATGGATGAAA |
GAPDH | NM_002046.2 | 55°C | F-5'ACAGTCAGCCGCATCTTCTT R-5'GACAAGCTTCCCGTTCTCAG |
ACTB | NM_001101.2 | 55°C | F-5'CTCTTCCAGCCTTCCTTCCT F-5'AGCACTGTGTTGGCGTACAG |
HPRT1 | NM_000194.1 | 55°C | F-5'AAGCTTGCTGGTGAAAAGGA R-5'AAGCAGATGGCCACAGAACT |
YWHAZ | NM_001135702.1 | 55°C | F-5'ACTTTTGGTACATTGTGGC R-5'CCGCCAGGAAAAACCAGT |
18S | NR_003286.2 | 60°C | F-5'AGGAATTCCCAGTAAGTGCG R-5'GCCTCACTAAACCATCCAA |
RP II | NM_000937.3 | 60°C | F-5'CTTCACGGTGCTGGGCATT R-5'GTGCGGCTGCTTCCATAA |
UBC | XM_002344708.1 | 60°C | F-5'CCTGGTGCTCCGTCTTAGAG R-5'TTTCCCAGCAAAGATCAACC |