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Figure 2 | BMC Molecular Biology

Figure 2

From: Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples

Figure 2

Determination of optimal number of reference genes for normalization by pairwise variation analysis using GeNorm software. Bar values indicate the magnitude of the change in normalization factor after the inclusion of an additional reference gene. A large variation indicates that the added gene has a significant effect and should probably be included for calculation of the normalization factor. a) n = 9, b) n = 19

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