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Figure 1 | BMC Molecular Biology

Figure 1

From: The leukemia associated ETO nuclear repressor gene is regulated by the GATA-1 transcription factor in erythroid/megakaryocytic cells

Figure 1

The sequence of the 5'flanking region of ETO. (A) Alignment with genomic DNA of ETO cDNA from HEL cells amplified by RLM-RACE. Nucleotide +1 indicates the translational start site (ATG). The transcription start site is shown to be at -318 bp. (B) The sequence of the 5'flanking region of the ETO promoter region was amplified by PCR with genomic DNA as template. Putative consensus binding sites for transcription factors identified with MatInspector http://www.genomatix.de/matinspector.html are marked. Alignment of the human sequence with mouse and rat genomic sequences showed a region at -659 to -432 bp (underlined) to be highly conserved suggesting the presence of a proximal promoter.

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