Effect of starvation on TGF-β6 gene expression in white muscle. Juvenile fish were starved for 5 days and then injected with PBS or GH (1 μg/gr body weight) or fed 2% of their body weight. All fish were sacrificed six hours after injections. Total RNA from white muscle of experimental fish was reverse-transcribed using oligo(dT) and subjected to PCR amplification of TGF-β6. β-actin was used as a control for equal amounts used in RT-PCR. Amplified products were analyzed on 1.5-2% agarose gels (A) and photographed under UV illumination following staining with ethidium bromide and then a densitometric analysis was performed (B). The expression of TGF-β6 was compared to that of another member of the TGF-β family, MSTN that is involved in muscle growth. The results are presented as a ratio to that of β-actin. Different letters indicates significant difference (P < 0.05).