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Table 4 Ranking output of the six reference genes according to their expression stability.

From: Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes

Experiment Organ Treatment ACTB1 EF1A1 HPRT RPL7 Tubb2C UbcE
    N G B N G B N G B N G B N G B N G B
Development Larva Dev1 5 4 5 1 1 1 4 5 3 6 3 6 2 6 4 3 2 2
   Dev2 6 6 6 3 2 2 4 3 4 1 1 1 5 5 5 2 4 3
Adult All None 6 6 6 3 1 1 4 3 3 2 2 2 5 5 5 1 4 4
Infection All Mock 6 6 6 1 1 1 4 3 3 3 2 2 5 5 5 2 4 4
   NNV 6 6 6 1 1 1 4 3 3 2 2 2 5 5 5 3 4 4
  Brain Mock 6 6 5 5 4 2 2 2 3 4 5 6 3 3 4 1 1 1
   NNV 6 6 6 3 3 3 2 1 2 4 4 5 5 5 4 1 2 1
  Eye Mock 6 6 6 3 1 1 1 3 2 4 2 3 5 5 5 2 4 4
   NNV 6 6 6 3 3 1 1 1 2 4 4 4 5 5 5 2 2 3
  Thymus Mock 6 6 5 3 3 4 1 2 1 5 4 6 4 5 3 2 1 2
   NNV 6 6 6 5 3 4 1 4 1 4 5 5 3 2 3 2 1 2
  Spleen Mock 6 6 6 4 2 3 5 3 4 2 1 5 3 5 2 1 4 1
   NNV 6 6 6 2 1 3 4 4 2 3 2 4 5 5 5 1 3 1
  Ant. kidney Mock 6 6 6 3 1 3 5 4 5 2 3 4 4 5 2 1 2 1
   NNV 6 6 6 2 1 4 5 5 5 4 2 1 3 4 3 1 3 2
  Liver Mock 6 6 6 3 2 2 4 3 4 2 1 1 5 5 5 1 4 3
   NNV 6 6 6 3 2 2 4 3 4 2 1 1 5 5 5 1 4 3
Leucocytes A. kidney All 4 4 5 5 2 1 2 5 4 1 3 3 6 6 6 3 1 2
  1. The rankings are calculated by NormFinder (N), geNorm (G), BestKeeper (B). A ranking of 1 is considered the best candidate, while 6 is listed as the worst. Abbreviations: Dev1 - developmental stages before activation of zygotic transcription, Dev2 - developmental stages after hatching, A. kidney - anterior kidney, Mock - mock-injected, NNV - NNV-injected.