Table 4 Ranking output of the six reference genes according to their expression stability.
Experiment | Organ | Treatment | ACTB1 | EF1A1 | HPRT | RPL7 | Tubb2C | UbcE | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
N | G | B | N | G | B | N | G | B | N | G | B | N | G | B | N | G | B | |||
Development | Larva | Dev1 | 5 | 4 | 5 | 1 | 1 | 1 | 4 | 5 | 3 | 6 | 3 | 6 | 2 | 6 | 4 | 3 | 2 | 2 |
Dev2 | 6 | 6 | 6 | 3 | 2 | 2 | 4 | 3 | 4 | 1 | 1 | 1 | 5 | 5 | 5 | 2 | 4 | 3 | ||
Adult | All | None | 6 | 6 | 6 | 3 | 1 | 1 | 4 | 3 | 3 | 2 | 2 | 2 | 5 | 5 | 5 | 1 | 4 | 4 |
Infection | All | Mock | 6 | 6 | 6 | 1 | 1 | 1 | 4 | 3 | 3 | 3 | 2 | 2 | 5 | 5 | 5 | 2 | 4 | 4 |
NNV | 6 | 6 | 6 | 1 | 1 | 1 | 4 | 3 | 3 | 2 | 2 | 2 | 5 | 5 | 5 | 3 | 4 | 4 | ||
Brain | Mock | 6 | 6 | 5 | 5 | 4 | 2 | 2 | 2 | 3 | 4 | 5 | 6 | 3 | 3 | 4 | 1 | 1 | 1 | |
NNV | 6 | 6 | 6 | 3 | 3 | 3 | 2 | 1 | 2 | 4 | 4 | 5 | 5 | 5 | 4 | 1 | 2 | 1 | ||
Eye | Mock | 6 | 6 | 6 | 3 | 1 | 1 | 1 | 3 | 2 | 4 | 2 | 3 | 5 | 5 | 5 | 2 | 4 | 4 | |
NNV | 6 | 6 | 6 | 3 | 3 | 1 | 1 | 1 | 2 | 4 | 4 | 4 | 5 | 5 | 5 | 2 | 2 | 3 | ||
Thymus | Mock | 6 | 6 | 5 | 3 | 3 | 4 | 1 | 2 | 1 | 5 | 4 | 6 | 4 | 5 | 3 | 2 | 1 | 2 | |
NNV | 6 | 6 | 6 | 5 | 3 | 4 | 1 | 4 | 1 | 4 | 5 | 5 | 3 | 2 | 3 | 2 | 1 | 2 | ||
Spleen | Mock | 6 | 6 | 6 | 4 | 2 | 3 | 5 | 3 | 4 | 2 | 1 | 5 | 3 | 5 | 2 | 1 | 4 | 1 | |
NNV | 6 | 6 | 6 | 2 | 1 | 3 | 4 | 4 | 2 | 3 | 2 | 4 | 5 | 5 | 5 | 1 | 3 | 1 | ||
Ant. kidney | Mock | 6 | 6 | 6 | 3 | 1 | 3 | 5 | 4 | 5 | 2 | 3 | 4 | 4 | 5 | 2 | 1 | 2 | 1 | |
NNV | 6 | 6 | 6 | 2 | 1 | 4 | 5 | 5 | 5 | 4 | 2 | 1 | 3 | 4 | 3 | 1 | 3 | 2 | ||
Liver | Mock | 6 | 6 | 6 | 3 | 2 | 2 | 4 | 3 | 4 | 2 | 1 | 1 | 5 | 5 | 5 | 1 | 4 | 3 | |
NNV | 6 | 6 | 6 | 3 | 2 | 2 | 4 | 3 | 4 | 2 | 1 | 1 | 5 | 5 | 5 | 1 | 4 | 3 | ||
Leucocytes | A. kidney | All | 4 | 4 | 5 | 5 | 2 | 1 | 2 | 5 | 4 | 1 | 3 | 3 | 6 | 6 | 6 | 3 | 1 | 2 |