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Table 3 Average Ct values ± SD of the six reference genes in the different experimental setups.

From: Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes

Experiment Sample Treatment ACTB1 EF1A1 HPRT RPL7 Tubb2C UbcE
Development Larva Dev1 23.7 ± 0.6 21.9 ± 0.3 24.2 ± 0.7 22.8 ± 0.4 25.8 ± 0.8 23.7 ± 0.3
   Dev2 21.3 ± 1.5 16.5 ± 0.7 22.3 ± 0.8 17.2 ± 0.6 20.5 ± 0.9 22.9 ± 0.8
Adult All None 21.2 ± 1.7 17.0 ± 0.5 22.3 ± 0.7 19.2 ± 0.6 20.7 ± 1.1 23.4 ± 0.8
Infection All Mock 21.0 ± 2.5 16.5 ± 0.7 21.4 ± 0.8 17.5 ± 0.8 19.9 ± 1.3 22.2 ± 0.9
   NNV 21.0 ± 2.5 16.5 ± 0.8 21.4 ± 0.8 17.4 ± 0.8 19.8 ± 1.2 22.1 ± 0.9
  Brain Mock 24.1 ± 0.7 17.2 ± 0.3 21.5 ± 0.3 18.4 ± 0.4 18.5 ± 0.3 22.2 ± 0.3
   NNV 23.9 ± 1.2 17.3 ± 0.5 21.5 ± 0.4 18.3 ± 0.5 18.6 ± 0.6 22.1 ± 0.4
  Eye Mock 22.6 ± 1.0 17.3 ± 0.4 22.8 ± 0.4 18.3 ± 0.4 19.8 ± 0.4 22.3 ± 0.3
   NNV 22.7 ± 0.9 17.3 ± 0.5 22.8 ± 0.4 18.3 ± 0.4 19.9 ± 0.5 22.3 ± 0.4
  Thymus Mock 19.0 ± 0.6 16.0 ± 0.3 20.9 ± 0.3 16.9 ± 0.3 19.0 ± 0.4 21.4 ± 0.3
   NNV 19.1 ± 1.0 16.1 ± 0.5 20.9 ± 0.6 16.9 ± 0.5 19.0 ± 0.6 21.4 ± 0.5
  Spleen Mock 18.7 ± 1.0 16.0 ± 0.5 21.3 ± 0.6 16.9 ± 0.4 20.5 ± 0.8 21.8 ± 0.7
   NNV 18.9 ± 1.6 16.0 ± 0.5 21.2 ± 0.7 16.9 ± 0.7 20.2 ± 0.9 21.6 ± 0.7
  A. kidney Mock 18.6 ± 1.1 15.8 ± 0.5 21.1 ± 0.4 16.7 ± 0.4 19.7 ± 0.8 21.8 ± 0.7
   NNV 18.5 ± 0.7 15.8 ± 0.4 21.1 ± 0.6 16.7 ± 0.6 19.6 ± 0.6 21.6 ± 0.5
  Liver Mock 23.2 ± 1.3 16.8 ± 0.5 20.9 ± 0.5 17.5 ± 0.5 21.7 ± 1.2 23.7 ± 0.7
   NNV 23.0 ± 1.2 16.6 ± 0.4 20.8 ± 0.4 17.5 ± 0.5 21.6 ± 1.1 23.5 ± 0.7
Leucocytes A. kidney Mock 24.4 ± 1.1 18.9 ± 1.0 27.8 ± 0.8 19.9 ± 0.8 24.5 ± 0.8 24.0 ± 0.9
   NNV 24.1 ± 1.0 18.6 ± 0.7 27.0 ± 1.1 19.5 ± 0.6 23.8 ± 1.2 23.6 ± 0.7
   Control 24.2 ± 1.1 18.4 ± 0.6 27.1 ± 1.1 19.4 ± 0.6 23.8 ± 1.1 23.3 ± 0.5
   ConAPMA 24.4 ± 1.1 19.1 ± 0.9 27.8 ± 1.0 20.0 ± 0.7 24.4 ± 1.0 24.2 ± 0.7
  1. Abbreviations: Dev 1 - developmental stages before activation of zygotic transcription, Dev 2 - developmental stages after hatching, A. kidney - anterior kidney, Mock - mock-injected, NNV - NNV-injected, Control - non-stimulated control cells, ConA-PMA - cells in vitro stimulated with ConA-PMA.