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Table 3 Average Ct values ± SD of the six reference genes in the different experimental setups.

From: Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes

Experiment

Sample

Treatment

ACTB1

EF1A1

HPRT

RPL7

Tubb2C

UbcE

Development

Larva

Dev1

23.7 ± 0.6

21.9 ± 0.3

24.2 ± 0.7

22.8 ± 0.4

25.8 ± 0.8

23.7 ± 0.3

  

Dev2

21.3 ± 1.5

16.5 ± 0.7

22.3 ± 0.8

17.2 ± 0.6

20.5 ± 0.9

22.9 ± 0.8

Adult

All

None

21.2 ± 1.7

17.0 ± 0.5

22.3 ± 0.7

19.2 ± 0.6

20.7 ± 1.1

23.4 ± 0.8

Infection

All

Mock

21.0 ± 2.5

16.5 ± 0.7

21.4 ± 0.8

17.5 ± 0.8

19.9 ± 1.3

22.2 ± 0.9

  

NNV

21.0 ± 2.5

16.5 ± 0.8

21.4 ± 0.8

17.4 ± 0.8

19.8 ± 1.2

22.1 ± 0.9

 

Brain

Mock

24.1 ± 0.7

17.2 ± 0.3

21.5 ± 0.3

18.4 ± 0.4

18.5 ± 0.3

22.2 ± 0.3

  

NNV

23.9 ± 1.2

17.3 ± 0.5

21.5 ± 0.4

18.3 ± 0.5

18.6 ± 0.6

22.1 ± 0.4

 

Eye

Mock

22.6 ± 1.0

17.3 ± 0.4

22.8 ± 0.4

18.3 ± 0.4

19.8 ± 0.4

22.3 ± 0.3

  

NNV

22.7 ± 0.9

17.3 ± 0.5

22.8 ± 0.4

18.3 ± 0.4

19.9 ± 0.5

22.3 ± 0.4

 

Thymus

Mock

19.0 ± 0.6

16.0 ± 0.3

20.9 ± 0.3

16.9 ± 0.3

19.0 ± 0.4

21.4 ± 0.3

  

NNV

19.1 ± 1.0

16.1 ± 0.5

20.9 ± 0.6

16.9 ± 0.5

19.0 ± 0.6

21.4 ± 0.5

 

Spleen

Mock

18.7 ± 1.0

16.0 ± 0.5

21.3 ± 0.6

16.9 ± 0.4

20.5 ± 0.8

21.8 ± 0.7

  

NNV

18.9 ± 1.6

16.0 ± 0.5

21.2 ± 0.7

16.9 ± 0.7

20.2 ± 0.9

21.6 ± 0.7

 

A. kidney

Mock

18.6 ± 1.1

15.8 ± 0.5

21.1 ± 0.4

16.7 ± 0.4

19.7 ± 0.8

21.8 ± 0.7

  

NNV

18.5 ± 0.7

15.8 ± 0.4

21.1 ± 0.6

16.7 ± 0.6

19.6 ± 0.6

21.6 ± 0.5

 

Liver

Mock

23.2 ± 1.3

16.8 ± 0.5

20.9 ± 0.5

17.5 ± 0.5

21.7 ± 1.2

23.7 ± 0.7

  

NNV

23.0 ± 1.2

16.6 ± 0.4

20.8 ± 0.4

17.5 ± 0.5

21.6 ± 1.1

23.5 ± 0.7

Leucocytes

A. kidney

Mock

24.4 ± 1.1

18.9 ± 1.0

27.8 ± 0.8

19.9 ± 0.8

24.5 ± 0.8

24.0 ± 0.9

  

NNV

24.1 ± 1.0

18.6 ± 0.7

27.0 ± 1.1

19.5 ± 0.6

23.8 ± 1.2

23.6 ± 0.7

  

Control

24.2 ± 1.1

18.4 ± 0.6

27.1 ± 1.1

19.4 ± 0.6

23.8 ± 1.1

23.3 ± 0.5

  

ConAPMA

24.4 ± 1.1

19.1 ± 0.9

27.8 ± 1.0

20.0 ± 0.7

24.4 ± 1.0

24.2 ± 0.7

  1. Abbreviations: Dev 1 - developmental stages before activation of zygotic transcription, Dev 2 - developmental stages after hatching, A. kidney - anterior kidney, Mock - mock-injected, NNV - NNV-injected, Control - non-stimulated control cells, ConA-PMA - cells in vitro stimulated with ConA-PMA.