The ratios of ESTs between 3'-AS at major and minor sites was plotted respective to their positions. The Y-axis indicates the distribution of ratio of ESTs between major and minor sites. The X-axis denotes the nucleotide distance between alternative and dominant splice sites. Alternative 3' splice sites upstream from the dominant splice site (red line) denote as negative, those downstream are positive. Pink dashed line denotes the alternative splices within ten nucleotides of the dominant site.