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Table 4 Primer sequences for generation of the 5' UTR constructs

From: Genetic variations regulate alternative splicing in the 5' untranslated regions of the mouse glioma-associated oncogene 1, Gli1

 

Forward primer

Reverse primer

L, LΔ1B

5'-GCGGCTAGC AGTAGGCAGTATAGGGTCC

5'-GCGGCTAGC GGCGTCTCAGGGAAGGAT

M, MΔ1B

5'-GCGGCTAGC TCCACCCAGCTCATCTTCTAG

5'-GCGGCTAGC GGCGTCTCAGGGAAGGAT

S, SΔ1B

5'-GCGGCTAGC CCAGCCCAGTTTCCAGCC

5'-GCGGCTAGC GGCGTCTCAGGGAAGGAT

  1. The NheI restriction sites are shown in Italics.