From: Genetic variations regulate alternative splicing in the 5' untranslated regions of the mouse glioma-associated oncogene 1, Gli1
Forward primers in exon 1
Reverse primers in exon 4
1st PCR
5'-AGTTTCCAGCCCTGGACCAC
5'-GAGGTCCGGATTACGGTTT
Nested PCR
5'-ACCGCGCCCCGACGGAG
5'-ATCAGAAAGGGGCGAGATGG