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Figure 4 | BMC Molecular Biology

Figure 4

From: Deficiency of the dual ubiquitin/SUMO ligase Topors results in genetic instability and an increased rate of malignancy in mice

Figure 4

Cellular transformation and chromosomal instability in Topors-deficient primary murine embryonic fibroblasts. A. Formation of foci by Topors-/- pMEFs after 3 weeks of growth. The upper section shows two representative plates for Topors+/+ and Topors-/- pMEFs. The lower section shows 10X-magnified images, including a focus in Topors-/- pMEFs. B. Anchorage-independent growth by Topors-/- pMEFs. 5 × 104 cells were seeded in soft agar and colonies assessed after 2 weeks of growth. Shown are two of four colonies detected in Topors-/- pMEF cultures. No colonies were observed in wild-type cultures. C. Distribution of chromosome numbers for metaphases analyzed in two independent Topors+/+ (n = 15), Topors-/- (n = 23), and Topors+/- (n = 25) early passage pMEF cell lines. Each bar represents a single metaphase. The difference between aneuploidy frequency in Topors+/+ versus Topors-/- pMEFs is statistically significant (p = 0.016). D. Representative karyotype of an early passage Topors-/- pMEF cell, showing trisomy for chromosome 1.

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