Skip to main content

Table 1 Induction of BTG-1 and RCAN1.1 upon DEX treatment of CEM cells

From: Quantitative polymerase chain reaction analysis by deconvolution of internal standard

  CEM-C1-15 cells CEM-C7-14 cells
Gene (Method)(*) - DEX + DEX - DEX + DEX
BTG-1 (Absolute) 6.5 × 104 copies
(± 7.3 × 103)
n = 2
4.4 × 105 copies
(± 1.4 × 105)
n = 3
4.9 × 104 copies
(± 1.2 × 103)
n = 2
7.1 × 105 copies
(± 1.2 × 105)
n = 2
β-actin
(Absolute)
1.4 × 108 copies
(± 1.4 × 107)
n = 2
1.4 × 108 copies
(± 2.7 × 107)
n = 3
1.3 × 108 copies
(± 1.6 × 107)
n = 2
8.5 × 107 copies
(± 5.8 × 106)
n = 2
BTG-1
(Relative)
0.047% of actin
(± 0.0072%)
0.31% of actin
(± 0.12%)
0.037% of actin
(± 0.0044%)
0.84% of actin
(± 0.16%)
BTG-1
(Normalized)
1x 6.6x
(± 0.40)
1x 23x
(± 0.22)
RCAN1.1 (Absolute) 1.4 × 105 copies
(± 2.7 × 103)
n = 2
5.1 × 105 copies
(± 4.5 × 103)
n = 2
2.0 × 105 copies
(± 1.8 × 104)
n = 2
1.3 × 106 copies
(± 3.0 × 104)
n = 2
β-actin
(Absolute)
1.0 × 108 copies
(± 2.5 × 107)
n = 2
7.3 × 107 copies
(± 2.3 × 107)
n = 2
1.9 × 108 copies
(± 3.0 × 106)
n = 2
8.5 × 107 copies
(± 3.4 × 107)
n = 2
RCAN1.1 (Relative) 0.14% of actin
(± 0.034%)
0.69% of actin
(± 0.22%)
0.11% of actin
(± 0.010%)
1.5% of actin
(± 0.61%)
RCAN1.1 (Normalized) 1x 5.1x
(± 0.40)
1x 14x
(± 0.41)
  1. (*) Average copy numbers (± s.d.) of template in cDNA generated from 200 ng poly(A)+ RNA. Relative copy numbers are given with respect to the 130 bp β-actin amplicon. Normalized values of BTG-1 and RCAN1.1 indicate the consequences of DEX treatment.