Table 1 Induction of BTG-1 and RCAN1.1 upon DEX treatment of CEM cells
From: Quantitative polymerase chain reaction analysis by deconvolution of internal standard
| CEM-C1-15 cells | CEM-C7-14 cells | |||
|---|---|---|---|---|
| Gene (Method)(*) | - DEX | + DEX | - DEX | + DEX |
| BTG-1 (Absolute) |
6.5 × 104 copies (± 7.3 × 103) n = 2 |
4.4 × 105 copies (± 1.4 × 105) n = 3 |
4.9 × 104 copies (± 1.2 × 103) n = 2 |
7.1 × 105 copies (± 1.2 × 105) n = 2 |
|
β-actin (Absolute) |
1.4 × 108 copies (± 1.4 × 107) n = 2 |
1.4 × 108 copies (± 2.7 × 107) n = 3 |
1.3 × 108 copies (± 1.6 × 107) n = 2 |
8.5 × 107 copies (± 5.8 × 106) n = 2 |
|
BTG-1 (Relative) |
0.047% of actin (± 0.0072%) |
0.31% of actin (± 0.12%) |
0.037% of actin (± 0.0044%) |
0.84% of actin (± 0.16%) |
|
BTG-1 (Normalized) | 1x |
6.6x (± 0.40) | 1x |
23x (± 0.22) |
| RCAN1.1 (Absolute) |
1.4 × 105 copies (± 2.7 × 103) n = 2 |
5.1 × 105 copies (± 4.5 × 103) n = 2 |
2.0 × 105 copies (± 1.8 × 104) n = 2 |
1.3 × 106 copies (± 3.0 × 104) n = 2 |
|
β-actin (Absolute) |
1.0 × 108 copies (± 2.5 × 107) n = 2 |
7.3 × 107 copies (± 2.3 × 107) n = 2 |
1.9 × 108 copies (± 3.0 × 106) n = 2 |
8.5 × 107 copies (± 3.4 × 107) n = 2 |
| RCAN1.1 (Relative) |
0.14% of actin (± 0.034%) |
0.69% of actin (± 0.22%) |
0.11% of actin (± 0.010%) |
1.5% of actin (± 0.61%) |
| RCAN1.1 (Normalized) | 1x |
5.1x (± 0.40) | 1x |
14x (± 0.41) |