Figure 5

TACC1 participates to the modulation of transcriptional activity by nuclear receptors. (A) The protein levels of TACC1 in control (non-targeting siRNA) or TACC1 siRNA treated cells were determined by immunoblotting with an anti-TACC1 antibody (Upstate). β-tubulin was used as a loading control. (B, C, D, E) Non-targeting siRNA or TACC1 siRNA treated HEK-293F cells were transiently transfected with TRE-TKLuc and TRα1 cDNA expression vector (B), RARE-TKLuc (C), Oct4-Binding Domain-Luc and Oct4 and Sox2 expression plasmids (D) or 5xcoll TATA Luc (E). Cells were treated with 10^-7M of T3 (B) or all-trans RA (ATRA) (C) for 48 h. Firefly luciferase was normalized for cotransfected Renilla luciferase activity (RLU). Open histogram, control siRNA treated cells; black histogram, TACC1 siRNA treated cells. The bars indicate the mean ± S.E. of triplicate transfections from a representative experiment (* p < 0.05, ** p < 0.01, by Student's-test).