Figure 3

Interaction of TACC1 or mutant TACC1 and TR in mammalian cells. (A) Flag-TACC1-J, Flag-TACC1-Jmut, Flag-TACC1-K or Flag-TACC1-Kmut (mut refering to mutations L609A and I610A) expression plasmids were cotransfected with TRα1 or TRα2 expression plasmids into COS-7 cells in the absence of T3 hormone. Forty-eight hours after transfection, whole cell lysates were prepared and immunoprecipitated with anti-TR antibody. Immunoprecipitates were resolved by SDS-PAGE and blotted with anti-flag and anti-TR antibodies. Inputs correspond to 1% of the proteins used for the coimmunoprecipitation assay. (B) Confocal microscopy analysis of the localization of co-transfected TRα2 and TACC1-K (wt) or TACC1-K mutant (mut) in COS-7 cells.