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Figure 1 | BMC Molecular Biology

Figure 1

From: The transforming acidic coiled coil (TACC1) protein modulates the transcriptional activity of the nuclear receptors TR and RAR

Figure 1

Representation of hTACC1 isoforms (A, J, K, S and the peptide Y11). Numbering represents the amino acid positions on the seminal human TACC1-A protein [7]. Each number points to the first amino acid of the following domain. The hatched region is the coiled-coil domain (202 Amino Acids (AA), from AA 604 to AA 805). For the nomenclature and schemes of the different isoforms, see [9, 13]. TACC1-K, -S and J proteins are natural variants. Compared to TACC1-A, TACC1-K protein is a novel isoform that lacks the exon 5 C-terminal domain; exon 5 in the mature mRNA is 121 bases long (see the sequence in TACC1-G, Genbank accession # BC041391). This short exon 5 appeared erroneously as a short exon 6 in TACC1-G and -J on Figure 6 in Lauffart et al. [31] (Ivan Still, pers. comm., with permission). The TACC1-S protein lacks domains corresponding to exons 2 and 3. The TACC1-J protein lacks the exon 2 and 3 domains and has the short exon 5 domain [31]. We found all four TACC1's in activated human RAJI cells; we found only TACC1-K and -J in mouse cells. TACC1-Y11 is the peptide found to interact with TRα2 in the yeast two-hybrid screen. Its mRNA contained the coding sequence of the C-terminal domain of the TACC1 protein, plus part of the 3'UTR sequence. The peptide begins at AA 491 and ends at AA 805. So the Y11 peptide (286 AA) corresponds mainly to the TACC1 coiled-coil domain. AA 525 to 553, corresponding to the C-terminal part of exon 5, are missing in Y11.

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