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Table 4 Primers used in this study.

From: Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks

Gene

Universal primers

Forward/Reverse

qPCR primers

Forward/Reverse

amplicon (bp)

E

actc1

-

CACCTTCCAGCAGATGTGGAT

TACTGGGCGGGACTGAGATT

101

0.93

dnaja2

YRTGCGBRTYATGATHAGACA

GACMSAVCCTGGYTCRATDACTTT

AAGAAGGTGGTGAAAGAAGTGAAGA

CAAAGTGCCTCAACAAGACCA

217

0.71

eef1b

-

GCTGTGGATGATGACGACGA

GCACCCCAAAGTAAGCCATC

247

0.90

hprt

GCTGAYCTVYTDGAYTACATCAA GGGCRTADCCYACMACRAAYTTGTC

TCAATCCCAATGACGGTAGACTT

AGTCTGCATTGTTTTACCCGTATC

161

0.83

mrip

GRGTRGAGAGYGGYTACTT

CARTCRAAKGTYTTBGAGCG

ACAGTACCGCAGGGCAAAGT

CCAATGCTTCTTCCACTGACC

126

0.92

polr2f

ATGTCNGAYAACGARGAYAAYTT

YTCRTCMMMNCCCCAGTC

TGATGGAGATGACTTTGACGATG

ACACGGGCTCGCTCATACTT

170

0.84

rpl6

-

CACCAGGAGGGCGAGATCT TGGTCATCCTTCCGCTGTTC

71

0.96

tubb2

*

CACCTTCATTGGCAACAGCAC

GGCCTCTGTGAACTCCATCTC

98

0.91

ubq

CYGGSAAGACCATCACCCT

GGTSGACTCYTTCTGGATGTTGTA

CTGGCAAACAGCTGGAAGA

ATCTGCATGCCACCTCTCA

99

1.01

  1. For part of the genes universal primers were used to acquire novel Epaulette shark sequences. The amplicon length and PCR efficiency (E) of qPCR primers is displayed.
  2. * qPCR primer designed directly from the tubb2 alignment, see table 1