Skip to main content

Advertisement

Table 2 Results of the mRNA expression stability analysis with NormFinder and GeNorm with ANOVA tests for differences among experimental groups.

From: Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks

Tissue/Rank NormFinder (Stability value)   GeNorm (M value)  
Cerebellum    
1 dnaja2** 0.043 eef1b* rpl6** 0.25
2 hprt** 0.045 ubq* 0.30
3 ubq* 0.061 dnaja2** 0.32
4 polr2f 0.067 hprt** 0.34
5 rpl6** 0.068 polr2f 0.37
6 eef1b* 0.070 tubb2** 0.40
7 tubb2** 0.079 mrip 0.45
8 mrip 0.121 actc1 0.49
9 actc1 0.122   
Heart     
1 eef1b 0.058 eef1b ubq 0.18
2 polr2f 0.070 hprt 0.21
3 mrip 0.080 polr2f 0.23
4 ubq 0.086 mrip 0.26
5 tubb2 0.093 tubb2 0.28
6 dnaja2 0.109 dnaja2 0.31
7 hprt 0.114 actc1 0.38
8 actc1 0.175 rpl6 0.45
9 rpl6 0.183   
Gills     
1 rpl6 0.044 eef1b rpl6 0.14
2 polr2f 0.064 polr2f 0.19
3 eef1b 0.066 dnaja2 0.23
4 tubb2 0.073 tubb2 0.24
5 ubq 0.084 hprt 0.26
6 dnaja2 0.084 ubq 0.28
7 mrip 0.089 mrip 0.29
8 hprt 0.111 actc1 0.51
9 actc1 0.277   
Eye     
1 ubq 0.087 eef1b rpl6 0.21
2 polr2f 0.110 hprt 0.42
3 tubb2 0.117 ubq 0.48
4 hprt 0.193 polr2f 0.54
5 actc1 0.210 actc1 0.58
6 eef1b 0.227 tubb2 0.62
7 rpl6 0.253 dnaja2 0.77
8 dnaja2 0.297 mrip 0.99
9 mrip 0.341   
  1. * ANOVA p < 0.05
  2. ** ANOVA p < 0.01