Table 1 Quality evaluation of total RNA samples
From: Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies
OD 260 nm/280 nm | 28S/18S area ratio | RNA integrity number (RIN) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
n | Mean | SD | CV (%) | Mean | SD | CV (%) | Mean | SD | CV (%) | ||
Mouse | Sham | 3 | 2.05 | 0.01 | 0.2 | 1.23 | 0.12 | 9.3 | 7.9 | 0.2 | 1.9 |
MI | 3 | 2.06 | 0.01 | 0.5 | 1.33 | 0.06 | 4.4 | 8.2 | 0.3 | 3.2 | |
Rat | Sham | 3 | 2.03 | 0.01 | 0.5 | 1.40 | 0.10 | 7.1 | 8.8 | 0.3 | 3.0 |
MI | 3 | 2.05 | 0.01 | 0.3 | 1.50 | 0.00 | 0.0 | 8.5 | 1.4 | 16.5 | |
Human | Donor | 4 | 2.03 | 0.03 | 1.3 | 1.36 | 0.36 | 26.5 | 7.2 | 1.9 | 26.0 |
Failing | 6 | 2.01 | 0.03 | 1.3 | 1.07 | 0.23 | 21.5 | 7.6 | 0.3 | 3.8 | |