Figure 4

Differentiation of F9 EC cells into PE is accompanied by specific decreases in expression of Brf1, Bdp1 and TBP proteins, as well as Brf1 and Bdp1 mRNAs. a Western blot of whole cell extract (50 μg) from untransfected F9 cells before (EC) or after (PE) differentiation and probed with antibodies against Brf1, Bdp1, TBP and actin, as indicated. b Western blot of whole cell extract (20 μg) from untransfected F9 cells before (EC) or after (PE) differentiation and probed with antibodies against laminin B1 and actin, as indicated. c Western blot of whole cell extract (50 μg) from untransfected F9 cells before (EC) or after (PE) differentiation and probed with antibodies against TFIIIC110 and actin, as indicated. d RT-PCR analysis to compare expression of mRNAs encoding Brf1, Bdp1, TBP and ARPP P0 in undifferentiated (EC) and differentiated (PE) F9 cells, as indicated.