Figure 3
Stable expression of Brf1 in F9 cells does not prevent down-regulation of tRNA gene transcription following differentiation. a Western blot of whole cell extract (50 μg) from untransfected F9 cells (lanes 1 and 2) and Brf1.F9 cells (lanes 3 and 4) before (EC) or after (PE) differentiation. Blots were probed with antibodies F-7 against HA and C-11 against actin, as indicated. b Quantitative comparison of tRNALeu gene transcription in vitro using extracts (20 μg) of Brf1.F9 and untransfected F9 cells before (EC) and after (PE) differentiation. Graph shows mean from three independent experiments +/- standard deviation. c Representative example of one of the transcription assays quantified in Fig. 3b.