Figure 2

Stable expression of Brf1 in F9 cells does not stimulate proliferation. a, b Proliferation curves showing numbers of viable Brf1.F9 (blue) and vec.F9 (red) cells, as determined by trypan blue staining, each day after plating 10⁴ undifferentiated EC cells (a) or differentiated PE cells (b). Counts were taken in triplicate and the results are presented as averages of three independent experiments +/- standard deviation. c RT-PCR analysis to compare expression of tRNA_i^Met and tRNA^Leu, and mRNAs encoding Brf1 and ARPP P0 in vec.F9 and Brf1.F9 cells, as indicated.