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Table 2 Primers for cloning

From: Identification of regulatory elements flanking human XIST reveals species differences

Fragment Content

Name

Sequence (5' to 3')

XIST promoter, positive control

0 F

TCGAGCTCCTTGCTCACCAATTGACTCGTAAG

 

0 R

CGGGTACCGAGGACGTGTCAAGAAGACACTAGG

1 kb upstream of XIST promoter

-1 kb F

TCGAGCTCCATTTCCACACTTGTAGAAACTTCTAGTAG

 

-1 kb R

CGGGTACCCTTACGAGTCAATTGGTGAGCAAG

2 kb upstream of XIST promoter

-2 kb F

TCGAGCTCGAGCCAAGCAGTAGTGAAGGTGA

 

-2 kb R

CGGGTACCGGTTGTCCTGGGTTTCTGTGA

3 kb upstream of XIST promoter

-3 kb F

TCGAGCTCCCCCGTGTTCTCTTTTGATAAACTAG

 

-3 kb R

CGGGTACCTCACCTTCACTACTGCTTGGCTC

65 kb upstream of XIST, covers HS 101

-65 kb F

TCGAGCTCGTGGAGTACCCTTTCTATCACAACT

 

-65 kb R

CGGGTACCTGGCTTGACTTCTAGGGTAAAGA

  1. * underlined is genomic sequence from NCBI; not underlined is adaptor (i.e. Sac I &Kpn I sites)