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Figure 1 | BMC Molecular Biology

Figure 1

From: Identification of regulatory elements flanking human XIST reveals species differences

Figure 1

Mapping DNase I hypersensitive sites downstream of XIST. A schematic of the mouse (A) and human (B) Xist/XIST genes and surrounding regulatory elements shows exons (black boxes) for Xist/XIST and other genes in the region (arrows indicate the direction of transcription). Grey dots show the location of conserved blocks between mouse and human [15], and triangles mark DNase I hypersensitive (DHS) sites (filled triangles - sites in undifferentiated cells; empty triangles - sites in differentiated cells; HS1, HS5 - HS7 [40], HS2 - HS4 [46, 47], HS8 - HS15 [55]). DNA from nuclei of ES-10, L1.10.1 and Xa and Xi-containing hybrids exposed to DNaseI was digested with restriction enzymes (Sca I (10 kb); Sap I/Blp I (10.2 kb); Nsi I (11.1 kb); Xmn I (9.3 kb); Nco I (11.1 kb) and Bam HI (6.4 kb)) for Southern analysis. All experiments were carried out at least twice and a representative Southern blot is shown for each fragment including a lane with no DNase I (parental fragment shown as black arrow) followed by six lanes of increasing amount of DNase I treatment. One HS site (grey arrow), was detected in the Sap I/Blp I fragment of ES-10 cells, with a size of ~7 kb. No HS site was detected in the Xmn I fragment which encompasses the four previously reported transcription start sites of TSIX [32]. No HS site was detected in either the Xa or Xi hybrid downstream of XIST.

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