Figure 5
Effects of octamer mutagenesis on expression levels. We performed substitutions of residues within the octamer in aqp-8's promoter region and assayed for changes in EC-expression. We targeted the -264G and -263C residues. The expression patterns were either not affected, diminished, or completely lost. A, aqp-8promoter::GFP reference strain. B, a -264G→A change led to no change in expression level. C, D, E, Mutations in the form of -264G→T, -263C→A, and -264GC→AG all lead to appreciable loss of GFP expression.