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Figure 1 | BMC Molecular Biology

Figure 1

From: Characterization of the octamer, a cis-regulatory element that modulates excretory cell gene-expression in Caenorhabditis elegans

Figure 1

Effects of the octamer at various distances upstream of a gene's translational start site. We placed the octamer upstream of various lengths of vit-2 promoter fragment to assay the ability of the octamer to drive EC-expression at different places within promoters. A, Octamers were appended onto the 5' end of decreasing vit-2 promoter-regions. The number represents the 5'-position of the vit-2 promoter fragment (distance upstream indicated). B, All vit-2 constructs less than and including the -392 bp (shown) construct failed to result in EC-expression although each of these constructs still have reporter expression in the intestine (I). C, Constructs with vit-2 upstream regions larger than and including the -448 bp (shown)construct had GFP expression in the EC (E) in addition to the expected expression in the intestine. The exception was the vit-2 promoter construct with the -652 bp 5'-end which failed to drive EC expression. * Both fluorescent images (B and C) were captured using 2 second exposure times.

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