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Figure 1 | BMC Molecular Biology

Figure 1

From: DNA-PKcs plays a dominant role in the regulation of H2AX phosphorylation in response to DNA damage and cell cycle progression

Figure 1

Depletion of DNA-PKcs inhibits DNA double-strand break (DSB) repair and sensitizes HeLa cells to ionizing radiation. A: Depletion of DNA-PKcs by specific siRNA in HeLa-H1 cells. B: Survival curves of DNA-PKcs-depleted cells (HeLa-H1) and control cells (HeLa-NC). C: Comet images of DNA DSB detected by neutral single cell gel electrophoresis 0-6 h post-irradiation. D: The repair kinetics of 4 Gy-induced DNA DSBs detected by comet assay. The tail moment was used as the endpoint of DNA DSB. Each bar represents the mean tail moment from three independent experiments. 100 individual comets were counted per time point for each experiment. # p < 0.01, as compared to the HeLa-NC cells at the same time point.

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