Skip to main content

Table 1 Variation and stability of the rpsO, rpsO Δ and rpsT transcripts as a function of Hfq quantity.

From: Hfq affects mRNA levels independently of degradation

 

rpsO mRNA (**)

rpsO Δ mRNA (**)

Strains

t1/2 (min) (*)

Relative RNA abundance at T = 0

t1/2 (min) (*)

Relative RNA abundance at T = 0

wt

0.98+/-0.12

100

  

hfq

1.79+/-0.13

10.3

  

wt/pGEM3

1.17+/-0.14

100

  

wt/pTX367

0.98+/-0.16

222

  

wt/pΔS15AUG

1.13+/-0.1

100

3.16+/-0.07

100

hfq/pΔS15AUG

2.09+/-0.12

50

4.67+/-0.09

62.5

 

rpsT mRNA

  

Strains

t1/2 (min) (*)

Relative RNA abundance at T = 0

  

wt P1-t1

1.31+/-0.17

100

  

wt P2-t1

1.57+/-0.17

100

  

hfq P1-t1

2.42+/-0.12

66.7

  

hfq P2-t1

5.43+/-0.07

16.9

  
  1. (*) Half-lives of transcripts were measured in strains N3433 (wt) and IBPC929 (hfq), transformed, when indicated, with pGEM3 empty vector, its derivative pTX367 expressing the hfq gene, or with plasmid pΔS15AUG bearing the constitutively expressed truncated rpsO gene.
  2. (**) The rpsO and the rpsO Δ transcripts originated from the chromosome and the pΔS15AUG plasmid, respectively.