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Table 3 Statistics results by BestKeeper software for seven selected genes based on Cp values

From: Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

 

H3

EF

ACT

NADH

rRNA

TUB

GAPDH

BI

n

15

15

15

15

15

15

15

15

GM (Cp)

26.59

19.29

24.39

26.65

7.09

21.12

29.24

20.38

S.D (± Cp)

0.74

1.10

1.11

1.22

1.24

2.14

2.29

1.16

CV (%Cp)

2.79

5.67

4.53

4.59

17.10

10.09

7.81

5.66

  1. n, number of samples; Cp, Crossing point cycle number equivalent terminology for Ct; GM(Cp), the geometric mean of Cp; S.D (± Cp), Cp standard deviation; CV (%Cp), variance coefficient expressed as percentage of Cp level; BI, BestKeeper Index.