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Figure 3 | BMC Molecular Biology

Figure 3

From: Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression

Figure 3

SRF and Elk-1 bind to the ZC3H12A promoter. A, Gel retardation assay with a fragment of ZC3H12A promoter (-95 - +67) containing the WT sequence or a mutated CArG box (ZC3H12A-mut) or a fragment of c-Fos promoter. The DNA was incubated with coreSRF. B and C, Chromatin immunoprecipitation of the transcription factors Elk-1 (B) and SRF (C) bound to the ZC3H12A promoter. HepG2 cells were incubated in 0.5% FCS medium for 12 hrs and then stimulated for 30 min with IL-1β. The antibodies anti-Elk-1 (B) or anti-SRF (C) were used to immunoprecipitate sonicated chromatin. As a negative control non-specific IgG were employed. Eluted DNA served as a template in PCR analysis with oligonucleotides specific for ZC3H12A promoter (top panel B and C) or EGR promoter (bottom panel B and C). 1% of input DNA is shown in lines 5 and 6. The panels show the inverted images of ethidium bromide-stained gels.

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