Figure 6
Non-normalised cycle threshold (C t ) of CXCL12, FABP1, MUC2 and PDCD4 in colorectal tissue. Using this approach, the expression of each gene appears to be down-regulated in tumours compared to normal tissues in the large cohort of patients (30 tumour and 34 normal tissue specimens), similar to previous published reports of reduced expression in colorectal tumours. No significant differences were noted in expression levels of target genes when using the small cohort of patients (10 tumour and 10 normal tissue specimens) (2-sample t-test). This confirms the effect of sample size on findings when using non-normalised Ct values and therefore the importance of normalisation especially in such type of studies