Figure 5

Relative quantity of target gene expression in colorectal tissues relative to each EC gene and to the geometric mean of the combined use of PPIA and B2M (PB). (A) Target gene expression in tumour versus normal using either individual candidate EC genes or the PB combination. (B) Significant differences in relative gene expression values as determined using ANOVA to compare mean expression levels across all tissues using either individual EC genes or PB in combination. (C) One way ANOVA indicating a reduction in the magnitude of error when the PB combination was used to normalise expression of CXCL12 (p < 0.001) and PDCD4 (p < 0.001) in comparison to the use of individual EC genes. See Table 1 Additional files for Post Hoc tests. Error bars indicate 95% confidence intervals.