Skip to main content

Table 3 Primers used for protein tagging

From: Correlation between ribonucleoside-diphosphate reductase and three replication proteins in Escherichia coli

Tagged protein

Primer direction

Primer sequence

NrdB

forward

GAAGTGGACACCGACGATTTGAGTAACAACCAGCTCGACTACAAAGACCATGACGG

 

reverse

TCAAATTTTTCCAATCGCCACTAATTGTTCCATGCACATCATATGAATATCCTCCTTAG

DnaB

forward

CGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATTCTATCCGTATGATGTTCC

 

reverse

GTGTTCCTTGATAAGTGTTTGCTTTAATTACCTAATATATGAATATCCTCCTTAG

DnaX

forward

GCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTTCTATCCGTATGATGTTCC

 

reverse

GGTTTCTCTCTCAATCACGTTAAGGATGACGAACGTATATGAATATCCTCCTTAG

  1. Underlined bases in forward primers correspond to the upstream of the epitope-encoding sequence.
  2. Underlined bases in reverse primers correspond to the 3'-end of the FRT sequence downstream of the kanamycin or chloramphenicol gene.
Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us