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Table 5 Copies of Fluc normalized to the copies of the reference genes Rluc, B2M and ActB

From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells

   Fluc/Rluc Fluc/B2M Fluc/ActB
   Normalized copies ± SD Normalized copies (× 10-3) ± SD Normalized copies (× 10-3) ± SD
pMN85 Untreated 3.91 0.53 2.73 0.34 1.41 0.23
  + Cd 5.11 1.00 2.85 0.46 1.86 0.25
pMN86 Untreated 3.50 0.51 2.22 0.43 1.22 0.31
  + Cd 211.35 19.46 155.77 29.88 86.30 12.42
  1. The number of copies of the genes (Fluc/Rluc/B2M/ActB) was calculated using standard curves generated as described in the Methods. The normalized copy numbers for Fluc/B2M and Fluc/ActB were multiplied by a factor of (1 × 103) to allow the comparison of the ratio of Fluc normalized to the chromosomal reference genes (B2M and ActB) to the ratio of Fluc normalized to the transiently co-transfected gene (Rluc). Abbreviations: SD- standard deviation