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Table 3 Comparison of the ranking of B2M and ActB, the selected chromosomal reference genes, and Rluc, present on a transiently co-transfected plasmid, based on geNorm, Normfinder and BestKeeper analyses

From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells

  geNorm Normfinder BestKeeper
  M value Ranking Stability value Standard error Ranking [r] p-value Ranking
ActB 0.44 1/2 1.342 0.515 3 0.931 0.001 2
B2M 0.44 1/2 0.432 0.955 1 0.985 0.007 1
Rluc 0.63 3 1.236 0.507 2 0.694 0.001 3
  1. Highlighted regions represent the best ranked gene in the analysis. Abbreviations: nd- not determined; M value- geNorm measure of expression stability, [r]- BestKeeper coefficient of correlation, p-value- BestKeeper probability value