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Table 2 Ranking of chromosomal reference genes based on geNorm, Normfinder and BestKeeper analyses

From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells

  geNorm Normfinder BestKeeper
  M value Ranking Stability value Standard error Ranking [r] p-value Ranking
18S 0.13 3 1.425 1.540 8 0.958 0.003 6
ActB 0.30 10 0.344 2.113 4 0.914 0.011 8
Atp5B 0.22 7 0.255 2.539 3 0.973 0.001 4
B2M 0.10 1/2 1.449 1.541 9 0.974 0.001 1/2/3
Cyc1 0.16 4 1.126 1.543 6 0.933 0.007 7
Eif4A2 0.24 8 0.204 2.981 2 0.974 0.001 1/2/3
GAPDH 0.20 6 0.444 1.872 5 0.881 0.020 9
RPL13A 0.19 5 0.195 3.090 1 0.974 0.001 1/2/3
SDHA 2.77 11 31.894 10.087 11 nd nd nd
TOP1 0.27 9 2.002 1.580 10 0.704 0.119 10
YWHAZ 0.10 1/2 1.225 1.539 7 0.959 0.002 5
  1. Highlighted regions represent the best ranked gene in the analysis. Abbreviations: nd- not determined; M value- geNorm measure of expression stability, [r]- BestKeeper coefficient of correlation, p-value- BestKeeper probability value