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Table 2 Ranking of chromosomal reference genes based on geNorm, Normfinder and BestKeeper analyses

From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells

 

geNorm

Normfinder

BestKeeper

 

M value

Ranking

Stability value

Standard error

Ranking

[r]

p-value

Ranking

18S

0.13

3

1.425

1.540

8

0.958

0.003

6

ActB

0.30

10

0.344

2.113

4

0.914

0.011

8

Atp5B

0.22

7

0.255

2.539

3

0.973

0.001

4

B2M

0.10

1/2

1.449

1.541

9

0.974

0.001

1/2/3

Cyc1

0.16

4

1.126

1.543

6

0.933

0.007

7

Eif4A2

0.24

8

0.204

2.981

2

0.974

0.001

1/2/3

GAPDH

0.20

6

0.444

1.872

5

0.881

0.020

9

RPL13A

0.19

5

0.195

3.090

1

0.974

0.001

1/2/3

SDHA

2.77

11

31.894

10.087

11

nd

nd

nd

TOP1

0.27

9

2.002

1.580

10

0.704

0.119

10

YWHAZ

0.10

1/2

1.225

1.539

7

0.959

0.002

5

  1. Highlighted regions represent the best ranked gene in the analysis. Abbreviations: nd- not determined; M value- geNorm measure of expression stability, [r]- BestKeeper coefficient of correlation, p-value- BestKeeper probability value