Figure 3From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cellsAverage number of copies of the Fluc, Rluc, B2M and ActB transcripts. HEK293 cells were transiently transfected with pMN85/pMN86 and pRL-SV40. The average numbers of copies represent those present in mRNA isolated from 0.2 μg of total RNA.Back to article page